Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 44 years) (ab43362)


  • Product nameHuman breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 44 years)
    See all Breast lysates
  • Description
    Human breast insoluble fraction
  • General notes

    Tissue: Breast Location: Left breast. Diagnosis: Infiltrating ductal carcinoma. Stage: IIB T2N1M0 Grade: II Sex: Female Age: 44 years Gross findings: Tumor size 4 cm., ill demarcated. Cut section firm and white. Ipsilateral axillary lymph nodes examined: 8 Microscopic findings: The tumor is composed of proliferation of malignant epithelial cells. The epithelial cells grown in the enlarged ducts contained comedo-necroses. The tumor cells have irregular, basophilic nuclei and abundant cytoplasm. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are abundant. Some areas show microinvasions. The stroma is infiltrated by numerous inflammatory cells. The lymph nodes show metastasis. 


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab41955: Extraction 1: soluble protein fraction ab43360 Human Breast Tumor Tissue lysate ab43359 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43362 Human Breast Tumor Tissue lysate ab43361 Human Breast Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 44 years) (ab43362)

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