Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 54 years) (ab43375)

Overview

  • Product nameHuman breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 54 years)
    See all Breast lysates
  • Description
    Human breast insoluble fraction
  • General notes

    Tissue: Breast Location: Left breast Diagnosis: Infiltrating ductal carcinoma Stage: IIB T2N1M0 Grade: II Gross findings: Tumor size 4 cm, ill demarcated. Cut section firm and white. Ipsilateral axillary lymph nodes examined: 12. Microscopic findings: The tumor is composed of proliferation of malignant epithelial cells. The epithelial cells are arranged as groups which have invaded the stroma. The tumor cells have irregular, basophilic nuclei and abundant cytoplasm. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are evident. The stroma is infiltrated by small numbers of chronic inflammatory cells. Necroses are seen but blood vessel invasions are not revealed. The lymph nodes show metastases (2/12).

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43375: Extraction 1: soluble protein fraction ab43373 Human Breast Tumor Tissue lysate ab43372 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43375 Human Breast Tumor Tissue lysate ab43374 Human Breast Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 54 years) (ab43375)

    ab43375 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"