Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 55 years) (ab43381)

Overview

  • Product nameHuman breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 55 years)
    See all Breast lysates
  • Description
    Hu breast insoluble fraction lysate
  • General notesTissue: Breast Location: Left breast. Diagnosis: Infiltrating ductal carcinoma. Stage: IIIB T4bN2M0 Grade: II Sex: Female Age: 55 years Gross findings: Tumor size 10 cm., ill demarcated. Cut section firm and brown/red. Insoluble protein fraction


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43381: Extraction 1: soluble protein fraction ab43380 Human breast tumor tissue lysate ab43379 Human breast normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab43381 Human breast tumor tissue lysate ab43382 Human breast normal tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 55 years) (ab43381)

    ab43381 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"