Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 75 years) (ab43488)

Overview

  • Product nameHuman breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 75 years)
    See all Breast lysates
  • Description
    Hu breast insoluble fraction lysate
  • General notesTissue: Breast Location: Left breast Diagnosis: Scirhous adenocarcinoma Stage: II T2N0M0 Grade: III Sex: Female Age: 75 years Gross findings: Tumor size 3 x 2.5 cm., ill demarcated. Cut section yellow/white. HISTOLOGIC PATTERN: Cell distribution: Diffuse: + Mosaic: - Necrosis: - Lymphocytic infiltration: + Vascular invasion: + Clusterized: - Alveolar formation: - Indian file: - Structure and Pattern: Streaming: - Storiform: - Fibrosis: + Pallisading: - Cystic degeneration: + Bleeding: - Myxoid change: - Psammoma body: - CELLULAR DIFFERENTIATION: Squamous: Squamoid: - Spindle: - Keratin: - Desmosome: - Pearl: - Adenomatous: Glandular cell: + Cell stratification: - Secretion: + Intracellular vacuole: + Glandular formation: + Sarcomatous: Round cell: - Spindle cell: - Leiomyoblast: - Lipoblast: - Rhadomyoblast: - NUCLEAR ATYPIA: Anisonucleosis: grade III Hyperchromatism: grade III Nucleolar prominent: grade III Multinucleated giant cell: grade III Mitotic activity: grade III Nuclear grade: grade III Insoluble protein fraction


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43488: Extraction 1: soluble protein fraction ab42046 Human Breast Tumor Tissue lysate ab42045 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43488 Human Breast Tumor Tissue lysate ab43487 Human Breast Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 75 years) (ab43488)

    ab43488 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Questions for ab43488.
    Please use the link above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"