Human breast tissue lysate (ductal carcinoma) - soluble fraction (female, 50 years) (ab42025)

Overview

  • Product nameHuman breast tissue lysate (ductal carcinoma) - soluble fraction (female, 50 years)
    See all Breast lysates
  • Description
    Human breast soluble fraction lysate
  • General notes

    Location: Right breast. Diagnosis: Invasive ductal carcinoma. Stage: IIB T2N1M0 Grade: III Gross findings: Tumor size 3 x 4 x 3 cm. Cut section hard and white/gray. Ipsilateral axillary lymph nodes examined: 9 Microscopic findings: Cancer in surgical margin of section: Negative Multifocal carcinoma: Negative Pectoral muscular/facia invasion: Negative Chest wall invasion: Negative Breast skin dermal invasion: Negative Dermal Lymphatic channel invasion: Negative Skin invasion with ulceration: Negative Dermal lymphatic channel invasion: Negative Skin satellite nodules: Negative Lymphatic and/or blood vessel invasion: Negative Tumor necrosis: Positive Number of nodes positive: 4 Tubule formation: 3 Nuclear pleomorphism: 3 Mitotic activity: 1 Lymphocytic response: Positive.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42025: Extraction 1: soluble protein fraction ab42025 Human Breast Tumor Tissue lysate ab42021 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab42027 Human Breast Tumor Tissue lysate ab42026 Human Breast Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: 0.0001% Pepstatin A, 0.0184% Sodium orthovanadate, 0.0042% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.0174% PMSF, 0.1% SDS, 0.0292% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate (ductal carcinoma) - soluble fraction (female, 50 years) (ab42025)

    ab42025 has not yet been referenced specifically in any publications.

    Product Wall

    We do carry a number of control lysates and tissue slides including those from breast tumor carcinoma. I have included links to these below. Breast tumor (human): ductal carcinoma (invasive) tissue slides (ab4697): http://www.abcam.com/breast-tumor-...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"