Human breast tissue lysate (ductal carcinoma) - soluble fraction (female, 52 years) (ab42029)


  • Product nameHuman breast tissue lysate (ductal carcinoma) - soluble fraction (female, 52 years)
    See all Breast lysates
  • Description
    Human breast soluble fraction lysate
  • General notesTissue: Breast Location: Left breast. Diagnosis: Infiltrating ductal carcinoma. Stage: IIIA T3N1M0 Grade: III Sex: Female Age: 52 years Gross findings: Tumor size 5 cm., ill demarcated. Cut section firm and white. Ipsilateral axillary lymph nodes examined: 9 Microscopic findings: The tumor is composed of proliferation of malignant epithelial cells. The epithelial cells are arranged in groups. The tumor cells have irregular , basophilic nuclei and abundant cytoplasm. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are evident. The stroma is infiltrated by small numbers of chronic inflammatory cells. Necroses are seen, but blood vessel invasions are not revealed. The lymph nodes show metastasis (6/9). Soluble protein fraction.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42029: Extraction 1: soluble protein fraction ab42029 Human Breast Tumor Tissue lysate ab42028 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab42031 Human Breast Tumor Tissue lysate ab42030 Human Breast Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate (ductal carcinoma) - soluble fraction (female, 52 years) (ab42029)

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