Human breast tissue lysate (ductal carcinoma) - soluble fraction (female, 60 years) (ab43544)

Overview

  • Product nameHuman breast tissue lysate (ductal carcinoma) - soluble fraction (female, 60 years)
    See all Breast lysates
  • Description
    Human breast soluble fraction lysate
  • General notes

    Tissue: Breast Location: Left breast Diagnosis: Infiltrating ductal carcinoma Stage: IIA T2N0M0 Grade: I Sex: Female Age: 60 years Gross findings: Tumor size 3cm. Cut section firm and white. Ipsilateral axillay lymph nodes examined : 3 Microscopic findings: Tumor composed of malignant epithelial cells, arranged in groups. They have irregular basophilic nucleic and abundant cytoplasm. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are evident. The stroma are infiltrated by small numbers of chronic inflammatory cells. Tumor necrosis positive. Blood vessel invasion negative.

     

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43544: Extraction 1: soluble protein fraction ab43544 Human Breast Tumor Tissue lysate ab43543 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43709 Human Breast Tumor Tissue lysate ab43708 Human Breast Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate (ductal carcinoma) - soluble fraction (female, 60 years) (ab43544)

    ab43544 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"