Human breast tissue lysate - insoluble fraction (female, 44 years) (ab43361)

Overview

  • Product nameHuman breast tissue lysate - insoluble fraction (female, 44 years)
    See all Breast lysates
  • General notesDiagnosis: Normal Sex: Female Age: 44 Insoluble protein fraction


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43361: Extraction 1: soluble protein fraction ab43360 Human Breast Tumor Tissue lysate ab43359 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43362 Human Breast Tumor Tissue lysate ab43361 Human Breast Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate - insoluble fraction (female, 44 years) (ab43361)

    ab43361 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Questions for ab43361.
    Please use the link above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"