Human breast tissue lysate (lobular carcinoma) - insoluble fraction (female, 39 years) (ab43725)


  • Product nameHuman breast tissue lysate (lobular carcinoma) - insoluble fraction (female, 39 years)
    See all Breast lysates
  • Description
    Human breast insoluble fraction
  • General notes

    Tissue: Breast Location: Left breast. Diagnosis: Infiltrating lobular carcinoma. Stage: III N2M0 Grade: Not graded. Sex: Female Age: 39 years Gross findings: Tumor size 6 cm., ill demarcated. Cut section firm and white. Microscopic findings: Biopsy shows the tumor cells are evidently malignant and invade into the surrounding connective tissues. The malignant cells are large, irregular in size and shape, with hyperchromatic nuclei and increased nucleo-cytoplasmic ratio, irregular nuclear. There is mucinous secretion in cells.


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43725: Extraction 1: soluble protein fraction ab43723 Human Lung Tumor Tissue lysate ab43722 Human Lung Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43725 Human Lung Tumor Tissue lysate ab43724 Human Lung Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tissue lysate (lobular carcinoma) - insoluble fraction (female, 39 years) (ab43725)

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