Human breast tumor tissue lysate (ductal carcinoma) - insoluble fraction (female, 47 years) (ab43451)


  • Product nameHuman breast tumor tissue lysate (ductal carcinoma) - insoluble fraction (female, 47 years)
    See all Breast lysates
  • Description
    Human breast insoluble fraction
  • General notes

    Tissue: Breast Location: Left breast Diagnosis: Bright Cell Carcinoma Stage: II T2NXMX Grade: II Sex: Female Age: 47 years Gross findings: Tumor size 3 x 4 cm, ill demarcated. Cut section form and gray/white. Histologic pattern: Cell distribution: +/- Diffuse: + Mosaic: - Necrosis: + Lymphocytic infiltration: - Vascular invasion: - Clusterized: + Alveolar formation: + Indian file: - Strucure/Pattern: +/- Streaming: - Storiform: - Fibrosis: + Pallisading: - Cystic degeneration: + Bleeding: + Myxoid change: + Psammoma body: - Cellular differentiation: Squamous: +/- Squamoid: - Spindle: - Keratin : - Desmosome: - Pearl: - Adenomatous: +/- Glandular cell: + Cell stratification: - Secretion: + Intracellular vacuole: + Glandular formation: - Sarcomatous: +/- Round cell: - Spindle cell: - Leiomyoblast: - Lipoblast: - Rhadomyoblast: - Nuclear atypia: 0 I II III Anisonucleosis: II Hyperchromatism: II Multinucleated giant cell: II Mototic activity: II Nuclear grade: II


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43451: Extraction 1: soluble protein fraction ab43449 Human Breast Tumor Tissue lysate ab43448 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43451 Human Breast Tumor Tissue lysate ab43450 Human Breast Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tumor tissue lysate (ductal carcinoma) - insoluble fraction (female, 47 years) (ab43451)

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