Human breast tumor tissue lysate (mucinous carcinoma) - insoluble fraction (female, 63 years) (ab43963)

Overview

  • Product nameHuman breast tumor tissue lysate (mucinous carcinoma) - insoluble fraction (female, 63 years)
    See all Breast lysates
  • Description
    Human breast insoluble fraction
  • General notes

    Tissue: Breast Location: Right breast Diagnosis: Mucinous carcinoma Stage: IIA T2N0M0 Grade: Not graded Gross findings: Tumor size 3 x 3 x 3 cm, ill demarcated. Cut section hard and gray/white. Ipsilateral axillary lymph nodes examined: 12 Microscopic findings: The tumor is composed of islands of epithelial cells with coarse chromatin. The tumor cells appear to be floating in the mucinous pool. The lymph nodes show negative.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43963: Extraction 1: soluble protein fraction ab43819 Human Breast Tumor Tissue lysate ab43811 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43963 Human Breast Tumor Tissue lysate ab43962 Human Breast Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human breast tumor tissue lysate (mucinous carcinoma) - insoluble fraction (female, 63 years) (ab43963)

    ab43963 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"