Abcam’s anti-Brucella IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Brucella in Human serum and plasma.
A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Brucella (antigen) Coated Microplate (12 x 8 wells)||12 Strips of 8 wells||1 unit|
|Brucella anti-IgG HRP Conjugate||Blue, Black cap||1 x 20ml|
|Brucella IgG Cut-off Control||Yellow, Green cap||1 x 3ml|
|Brucella IgG Negative Control||Yellow, Blue cap||1 x 2ml|
|Brucella IgG Positive Control||Yellow, Red cap||1 x 2ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108712 in the following tested applications.
|Indirect ELISA||Use at an assay dependent dilution.|