Overview

  • Product name
    Human Anti-Brucella IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 23 5.3%
    Pos.Serum 24 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 12 3.6%
    Pos.Serum 12 4.8%
  • Sample type
    Serum, Plasma
  • Assay type
    Indirect
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Brucella IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Brucella in Human serum and plasma.

     

    A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Protocols

References

This product has been referenced in:
  • Zhu J  et al. Lysine methylation represses p53 activity in teratocarcinoma cancer cells. Proc Natl Acad Sci U S A 113:9822-7 (2016). WB ; Human . Read more (PubMed: 27535933) »

See 1 Publication for this product

Customer reviews and Q&As

The positive and negative IgG controls in the assay are in fact yellow solutions (not the vials), due to the addition of the preservative KathonTM, also known generically as methylisothiazolinone. The concentration of the preservative is 0.1% w/v.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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