Human Anti-Brucella IgG ELISA Kit (ab108712)

Overview

  • Product nameHuman Anti-Brucella IgG ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 23 5.3%
    Pos.Serum 24 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 12 3.6%
    Pos.Serum 12 4.8%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Brucella IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Brucella in Human serum and plasma.

     

    A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab108712 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent dilution.

    Protocols

    References for Human Anti-Brucella IgG ELISA Kit (ab108712)

    ab108712 has not yet been referenced specifically in any publications.

    Product Wall

    The positive and negative IgG controls in the assay are in fact yellow solutions (not the vials), due to the addition of the preservative KathonTM, also known generically as methylisothiazolinone. The concentration of the preservative is 0.1% w/v.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"