Overview

  • Product name
    Human c-Kit ELISA Kit (CD117)
    See all c-Kit kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 5.629ng/ml 0.34 6%
    Sample B 8 1.231ng/ml 0.06 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 8 5.44ng/ml 0.43 7.9%
    Sample B 8 1.314ng/ml 0.1 7.6%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.095 ng/ml
  • Range
    0.312 ng/ml - 10 ng/ml
  • Assay time
    1h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s c-Kit Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of c-Kit in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of c-Kit present in the samples.

    This kit will recognize both endogenous and recombinant Human c-Kit.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated anti c-Kit 1 x 0.4ml 2 x 0.4ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    c-Kit Control Silver 2 vials 4 vials
    c-Kit Microplate (12 x 8 well strips) 1 unit 2 units
    c-Kit Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Plastic Plate Covers 1 x 2 units 1 x 2 units
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Relevance
    KIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
  • Cellular localization
    Cell Membrane; single-pass type I membrane protein
  • Alternative names
    • CD117
    • Mast/stem cell growth factor receptor precursor
    • PBT
    • Proto oncogene tyrosine protein kinase Kit
    • SCFR
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab45924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab45924.

Protocols

References

ab45924 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


The c-Kit Human ELISA Kit (ab45924) and Ferritin Human ELISA Kit (ab108698) both come with strip wells provided.

Thank you for your enquiry.

ab45925 is suitable for Cerebrospinal fluid.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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