Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids1 to 439

Specifications

Our Abpromise guarantee covers the use of ab84132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • FormLiquid
  • Additional Notes

    The c-Myc protein has a His-tag and is purified by nickel affinity column.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: Potassium chloride, 0.0154% DTT, Tris HCl, EDTA, 20% Glycerol

    Protein is stored in 20 mM Tris-Cl (pH 7.5), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.

General info

  • Alternative names
    • AU016757
    • Avian myelocytomatosis viral oncogene homolog
    • bHLHe39
    • c Myc
    • Cellular myelocytomatosis oncogene
    • Class E basic helix-loop-helix protein 39
    • MRTL
    • Myc
    • Myc protein
    • Myc proto oncogene protein
    • Myc proto-oncogene protein
    • myc-related translation/localization regulatory factor
    • MYC_HUMAN
    • Myc2
    • MYCC
    • Myelocytomatosis oncogene
    • Niard
    • Nird
    • Oncogene Myc
    • OTTHUMP00000158589
    • Proto-oncogene c-Myc
    • Protooncogene homologous to myelocytomatosis virus
    • RNCMYC
    • Transcription factor p64
    • Transcriptional regulator Myc-A
    • V-Myc avian myelocytomatosis viral oncogene homolog
    • v-myc myelocytomatosis viral oncogene homolog (avian)
    see all
  • FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
  • Information by UniProt

References for Human c-Myc full length protein (ab84132)

This product has been referenced in:
  • Tremblay R  et al. The development of a high-yield recombinant protein bioreactor through RNAi induced knockdown of ATP/ADP transporter in Solanum tuberosum. J Biotechnol 156:59-66 (2011). Read more (PubMed: 21864587) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. The concentration of ab84132 is 0.500 mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. I can confirm that ab84132 c-Myc protein (Human) has an 8x His-tag at its c-terminus and purified in a soluble (native) form by FPLC using both Nickel Sepharose and Superdex 200. I hope this information will be helpful...

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Application Western blot

Review text: Used us a positive control in western blot among ChIP samples.
Sample: Human Cell lysate - nuclear (colon)
Loading amount: 0.01 µg
Specification: colon
Gel Running Conditions: Reduced Denaturing (4-12%)
Blocking step: Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 4°C

Other product details
Dilution: 1/1000
Incubation time: 16 hour(s) and 0 minute(s) · Temperature: 4°C

Primary antibody (in addition to 'c-Myc protein (Human)')
Dilution: 1/1000

Secondary antibody
Name: Abcam antibody:(not specified yet)
Dilution: 1/1000

Detection
Detection method: ECL substrate
Exposure: 1 minute(s) and 0 second(s)
Bands: Specific: around 55 kDa
Negative control: BSA ChIPed cell lysate nuclear
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Verified customer

Submitted Aug 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"