Human C Reactive Protein ELISA Kit (CRP) (ab99995)

Overview

  • Product nameHuman C Reactive Protein ELISA Kit (CRP)
    See all C Reactive Protein kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    2.47 pg/ml - 600 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106.2 95% - 116%
    Serum 116.7 106% - 127%
    Plasma 102.2 93% - 112%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s C Reactive Protein (CRP) Human ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CRP in serum, plasma, cell culture supernatants.


    This assay employs an antibody specific for Human CRP coated on a 96-well plate. Standards and samples are pipetted into the wells and CRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.


     

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • Tissue specificityFound in plasma.
  • Sequence similaritiesBelongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Pentraxin 1, short
    • C reactive protein
    • C reactive protein pentraxin related
    • C-reactive protein(1-205)
    • CRP
    • CRP_HUMAN
    • MGC88244
    • Pentraxin 1
    • PTX 1
    • PTX1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab99995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human C Reactive Protein ELISA Kit (CRP) images

  • huCRP measured in biological fluids showing quantity (pg) per mL of sample tested.

  • Representative Standard Curve using ab99995.

Protocols

References for Human C Reactive Protein ELISA Kit (CRP) (ab99995)

ab99995 has not yet been referenced specifically in any publications.

Product Wall


The shelf life of each of our kits is indicated in the individual protocol booklet.

ab99995 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80&d...

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The lab sent the following comments and questions. Please let me know if you have the answers to those. I think the lab is trying to understand where exactly the problem might lie.

But I still think the standard curve should look better than w...

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Thank you so much for the additional information. I'll pass it on to the lab.

Should they have more suggestions, I will let you know.

I hope the 2 new kits will work as expected.

Please let me know if you have further questions.

Thank you for your emails.

I apologize that I could not get back to you earlier, but our office was closed over the holidays.

I am very sorry to hear that your effort and attempts to try to get the kits to work were unsuccessful. I wi...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your patience.
I have heard back from the lab today with the following response:

"I am not quite sure what exactly the issue is with the standard curve. I plotted the values in a linear regression model and curve looks f...

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Thank you for your phone message.

I have emailed the lab yesterday but have not yet heard back. I will follow up with them today.

As for the antibody vial:

Item F detection antibody comes as a very concentrated, small volume ...

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Thank you for your message. I am sorry to hear there was a shortage of the HRP-streptavidin concentrate item in the ab99995 kit.

If this has been purchased in the last 6 months, I will be pleased to provide a replacement of this component. I...

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Thank you for contacting us.

This is very normal, the buffer contains proteins which gets precipitated at low temperature. Please keep the buffer at room temperature for few minutes and then vortex it. The white opaque residue will go away. Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"