Overview

  • Product nameHuman C Reactive Protein ELISA Kit (PTX1)
    See all C Reactive Protein kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.25 ng/ml
  • Range
    0.5 ng/ml - 5 ng/ml
  • Recovery

    98.5 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s C Reactive Protein (PTX1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein concentrations in Human plasma, serum, saliva, milk, urine and cell culture supernatants.


    A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • Tissue specificityFound in plasma.
  • Sequence similaritiesBelongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Pentraxin 1, short
    • C reactive protein
    • C reactive protein pentraxin related
    • C-reactive protein(1-205)
    • CRP
    • CRP_HUMAN
    • MGC88244
    • Pentraxin 1
    • PTX 1
    • PTX1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human C Reactive Protein ELISA Kit (PTX1) images

  • Representative Standard Curve using ab108826

Protocols

References for Human C Reactive Protein ELISA Kit (PTX1) (ab108826)

This product has been referenced in:
  • Strøm Halden TA  et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). Read more (PubMed: 24452849) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

1. Your software may have a way to adjust the correction wavelength, or you can run it without the correction factor. The 570 wave length is subtracted to reduce the background noise and lower the OD. If you are unabl...

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I checked the details and can confirm that C Reactive Protein (PTX1) Human ELISA Kit ab108826 has been tested and covered by the guarantee for use in urine samples. Our apologies for any confusion.

I was not able to find any literature to...

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Thank you for your enquiry regarding these kits.

I would suggest reading through the Protocol Booklets for each of these kits provided on the product datasheets. These documents contain specific information about the sample types, sample prep...

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Thank you for your enquiry. I can confirm that both these kits ab46042 and ab108826 should be suitable for use with saliva samples. I can confirm that  kit ab108826 has been tested on saliva samples,  We suggest to use saliva samples at 1x (no d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"