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|Sample type||Average %||Range|
|Cell culture supernatant||115||106% - 123%|
|Saliva||117||107% - 130%|
|Milk||104||95% - 115%|
|Urine||88||78% - 103%|
|Serum||91||83% - 104%|
|Heparin Plasma||116||110% - 121%|
|EDTA Plasma||88||84% - 90%|
|Citrate Plasma||91||86% - 96%|
Abcam’s CA125 (MUC16) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human serum, plasma, cell culture supernatants, urine, milk, and saliva samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Samples diluted in Sample Diluent 25BS – 0.07 Units/mL
Samples diluted in Sample Diluent 50BS – 0.071 Units/mL
Cancer antigen 125 (CA125, also known as Mucin 16) is a membrane associated cell surface protein containing approximately 22,096 amino acids. CA125 is the largest member of the Mucin family of proteins and is highly o-glycosylated. The hydrophilic nature of mucin proteins gives them the ability to form protective/lubricating gel-like barriers to protect against chemotherapy agents, foreign particles and infectious agents. CA125 is used as a biomarker for ovarian cancer detection, with about 90% of women with advanced ovarian cancer having elevated levels of CA125 present in their blood serum.
|Components||1 x 96 tests|
|10X CA125 (MUC16) Capture Antibody||1 x 600µl|
|10X CA125 (MUC16) Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent CPI - HAMA Blocker (ab193969)||1 x 6ml|
|CA125 (MUC16) Human Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Sample Diluent 50BS||1 x 20ml|
|Sample Diluent NS (ab193972)||1 x 50ml|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab195213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
Non-background subtracted data from duplicate measurements are plotted. CA125 was detectable in HeLa cells culture supernatants (4-days culture), HepG2 and THP1 cell culture supernatants (4-days culture) were loaded as negative controls.
Duplicate interpolated values of CA125 (mean +/- SD, n = 2) are graphed. Medical histories of individual donors was not known. Measurements of CA125 are known to fluctuate in individual serums due to onset of certain cancers and benign conditions, thus CA125 elevation alone is not reason enough for diagnosis.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"