• Product name
    Human CA125 ELISA Kit (MUC16)
    See all Cancer Antigen CA125 kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 3 4.6%
    Sample n Mean SD CV%
    Overall 8 3.82%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.07 U/ml
  • Range
    1.56 U/ml - 100 U/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 115 106% - 123%
    Saliva 117 107% - 130%
    Milk 104 95% - 115%
    Urine 88 78% - 103%
    Serum 91 83% - 104%
    Heparin Plasma 116 110% - 121%
    EDTA Plasma 88 84% - 90%
    Citrate Plasma 91 86% - 96%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CA125 (MUC16) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human serum, plasma, cell culture supernatants, urine, milk, and saliva samples.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Samples diluted in Sample Diluent 25BS – 0.07 Units/mL
    Samples diluted in Sample Diluent 50BS – 0.071 Units/mL

  • Notes

    Cancer antigen 125 (CA125, also known as Mucin 16) is a membrane associated cell surface protein containing approximately 22,096 amino acids. CA125 is the largest member of the Mucin family of proteins and is highly o-glycosylated. The hydrophilic nature of mucin proteins gives them the ability to form protective/lubricating gel-like barriers to protect against chemotherapy agents, foreign particles and infectious agents. CA125 is used as a biomarker for ovarian cancer detection, with about 90% of women with advanced ovarian cancer having elevated levels of CA125 present in their blood serum.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X CA125 (MUC16) Capture Antibody 1 x 600µl
    10X CA125 (MUC16) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    CA125 (MUC16) Human Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • Research areas
  • Function
    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • Tissue specificity
    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • Sequence similarities
    Contains 2 ANK repeats.
    Contains 56 SEA domains.
  • Domain
    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • Post-translational
    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • Cellular localization
    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • Alternative names
    • CA 125
    • CA-125
    • CA125
    • MUC 16
    • MUC-16
    • MUC16
    • MUC16_HUMAN
    • Mucin 16
    • Mucin-16
    • Ovarian cancer related tumor marker CA125
    • Ovarian cancer-related tumor marker CA125
    • Ovarian carcinoma antigen CA125
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab195213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Background-subtracted data values (mean +/- SD) are graphed.

  • Non-background subtracted data from duplicate measurements are plotted. CA125 was detectable in HeLa cells culture supernatants (4-days culture), HepG2 and THP1 cell culture supernatants (4-days culture) were loaded as negative controls.

  • Duplicate interpolated values of CA125 (mean +/- SD, n = 2) are graphed. Medical histories of individual donors was not known. Measurements of CA125 are known to fluctuate in individual serums due to onset of certain cancers and benign conditions, thus CA125 elevation alone is not reason enough for diagnosis.



This product has been referenced in:
  • Pochechueva T  et al. Naturally occurring anti-glycan antibodies binding to Globo H-expressing cells identify ovarian cancer patients. J Ovarian Res 10:8 (2017). ELISA ; Human . Read more (PubMed: 28187738) »
  • Pochechueva T  et al. Blood Plasma-Derived Anti-Glycan Antibodies to Sialylated and Sulfated Glycans Identify Ovarian Cancer Patients. PLoS One 11:e0164230 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27764122) »

See all 2 Publications for this product

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