Abcam’s anti-Candida albicans IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Candida albicans in Human serum and plasma.
A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Candida albicans (IgG) Coated Microplate (12 x 8 wells)||1 unit|
|Candida albicans anti-IgG HRP Conjugate||Blue with Black cap||1 x 20ml|
|Candida albicans IgG Cut-off Control||Yellow with Green cap||1 x 3ml|
|Candida albicans IgG Negative Control||Yellow with Blue cap||1 x 2ml|
|Candida albicans IgG Positive Control||Yellow with Red cap||1 x 2ml|
|IgG Sample Diluent||Yellow with white cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108715 has not yet been referenced specifically in any publications.