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Abcam’s anti-Candida albicans IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Candida albicans in Human serum or plasma.
A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Candida albicans (IgM) Coated Microplate (12 x 8 wells)||1 unit|
|Candida albicans anti-IgM HRP Conjugate||Red Bottle, Black Cap||1 x 20ml|
|Candida albicans IgM Cut-off Control||Yellow Bottle, Green Cap||1 x 3ml|
|Candida albicans IgM Negative Control||Yellow Bottle, Blue Cap||1 x 2ml|
|Candida albicans IgM Positive Control||Yellow Bottle, Red Cap||1 x 2ml|
|Foil Cover||1 unit|
|IgM Sample Diluent||Green Bottle, White Cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip Holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108716 has not yet been referenced specifically in any publications.