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Abcam’s Carcino Embryonic Antigen in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Carcino Embryonic Antigen in Human serum.
A 96-well plate has been precoated with anti-Carcino Embryonic Antigen antibodies. Samples and standards and are added to the wells, where Carcino Embryonic Antigen in the sample and standards binds to the precoated antibody. Added Anti-Carcino Embryonic Antigen-HRP conjugate binds to the antibody-Carcino Embryonic Antigen complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Carcino Embryonic Antigen in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Carcino Embryonic Antigen Coated Microplate (12 x 8 wells)||1 unit|
|Anti-Carcino Embryonic Antigen HRP Conjugate||1 x 13ml|
|CEA Standard 0 - 0 ng/mL||1 x 1ml|
|CEA Standard 1 - 3 ng/mL||1 x 1ml|
|CEA Standard 2 - 12 ng/mL||1 x 1ml|
|CEA Standard 3 - 30 ng/mL||1 x 1ml|
|CEA Standard 4 - 60 ng/mL||1 x 1ml|
|CEA Standard 5 - 120 ng/mL||1 x 1ml|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab108635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Representative standard curve using ab108635.s
ab108635 has not yet been referenced specifically in any publications.