Overview

  • Product name
    Human Carcinoembryonic Antigen ELISA Kit (CD66e)
    See all Carcino Embryonic Antigen CEA kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    HeLa 5 4.4%
    Inter-assay
    Sample n Mean SD CV%
    HeLa 3 3.3%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.235 ng/ml
  • Range
    1 ng/ml - 65 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 103 94% - 118%
    Cell culture media 93 83% - 119%
    Heparin Plasma 97 94% - 99%
    EDTA Plasma 100 97% - 107%
    Citrate Plasma 94 89% - 101%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Carcinoembryonic Antigen (CEA/CD66e) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CEA/CD66e protein in human serum, plasma, cell culture supernatant, cell and tissue lysates.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm.Optionally,instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


     


    Sensitivity:


    Samples diluted in Sample Diluent NS - 700 pg/mL


    Samples diluted in Sample Diluent 10 BP - 623 pg/mL


    Samples diluted in 1X Cell Extraction Buffer - 235 pg/mL

  • Notes

    CEA/CD66E is endcoded by the CECAM5 gene which produces a cell adhesion molecule consisting of an Ig variable region-like amino terminal domain followed by up to six Ig constant region-like domains anchored to the cell membrane by either a glycosylphosphatidylinositol (GPI) moiety or a proteinaceous transmembrane and cytoplasmic domain. CEA is normally expressed on epithelial cells and is found abundantly on the apical surface of the gastrointestinal epithelium, as well as the nasopharynx, lung, urogenital tract, and sweat glands. Under physiologic conditions, only low amounts of soluble CEA can be detected in serum. However, in many different cancers, CEA is highly up regulated.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab183365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background subtracted data from duplicate measurements are plotted.

  • Cells were cultured for 2 days prior to collection of conditioned media supernatants. Background subtracted data from duplicate measurements are plotted. Quantitation of CEA secretion in HeLa and LoVo cells. Interpolated values of secreted CEA were calculated and multiplied by appropriate dilution factor.

Protocols

References

This product has been referenced in:
  • Sun Z  et al. Pre-operative to post-operative serum carcinoembryonic antigen ratio is a prognostic indicator in colorectal cancer. Oncotarget 8:54672-54682 (2017). Read more (PubMed: 28903373) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Sample: 5 ul of human serum
Incubation time: Samples with antibody mix were incubated in cold room overnight.
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Submitted Nov 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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