Human Cardiotrophin 1 ELISA Kit (CTF1) (ab99996)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 16.46 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cardiotrophin 1 ELISA Kit (CTF1)
See all CT-1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
16.46 pg/ml - 12000 pg/ml -
Recovery
100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.2 76% - 112% Plasma 101.2 70% - 134% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Cardiotrophin 1 (CTF1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cardiotrophin 1 in plasma and cell culture supernatants. (Human Cardiotrophin 1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Cardiotrophin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Cardiotrophin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cardiotrophin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cardiotrophin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human Cardiotrophin 1 in normal serum with ab99996, only in serum spiked with Human Cardiotrophin 1.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Human Cardiotrophin 1 2 vials Cardiotrophin 1 Microplate (12 x 8 wells) 1 unit Recombinant Cardiotrophin 1 Human Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. -
Tissue specificity
Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cardiophin 1
- Cardiophin1
- Cardiotrophin I
see all -
Database links
- Entrez Gene: 1489 Human
- Omim: 600435 Human
- SwissProt: Q16619 Human
- Unigene: 483811 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99996 has not yet been referenced specifically in any publications.