|Sample type||Average %||Range|
|Cell culture supernatant||98.2||76% - 112%|
|Plasma||101.2||70% - 134%|
Abcam’s Cardiotrophin 1 (CTF1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cardiotrophin 1 in plasma and cell culture supernatants. (Human Cardiotrophin 1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Cardiotrophin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Cardiotrophin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cardiotrophin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cardiotrophin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human Cardiotrophin 1 in normal serum with ab99996, only in serum spiked with Human Cardiotrophin 1.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|600X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated anti-Human Cardiotrophin 1||2 vials|
|Cardiotrophin 1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Cardiotrophin 1 Human Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab99996 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
Representative Standard Curve using ab99996.
Standard curve with background signal subtracted (duplicates; +/- SD).
ab99996 has not yet been referenced specifically in any publications.