Overview

  • Product name
    Human Caspase-1 ELISA Kit
    See all Caspase-1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    cell extract 3 4.4%
    Inter-assay
    Sample n Mean SD CV%
    cell extract 5 6.1%
  • Sample type
    Cell culture supernatant, Cell culture extracts, Tissue Extracts, Cell culture media
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    5.5 pg/ml
  • Range
    39.06 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 95 89% - 99%
    Tissue Extracts 86 83% - 90%
    Cell culture media 103 101% - 105%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Caspase-1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Caspase-1 protein in human cell culture supernatant, cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Caspase-1 is a thiol protease that cleaves IL-1 beta precursor releasing the mature cytokine which is involved in a variety of inflammatory processes. Caspase-1 is part of the inflammasome complex. It is important for defense against pathogens. Caspase-1 also cleaves and activates sterol regulatory element binding proteins (SREBPs). Caspase-1 can also promote apoptosis as it is part of caspase cascade.


    Sensitivity:
    Sample Diluent NS – 16.1 pg/mL
    1X Cell Extraction Buffer PTR – 5.5 pg/mL

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab219633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  •  Background-subtracted data values (mean +/- SD) are graphed.

  • Example of human Caspase-1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Caspase-1 were measured in duplicate and interpolated from the Caspase-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Caspase-1 concentration was determined to be 1497 pg/mL in LPS treated (8 hours) THP-1 cell extract, 1586 pg/mL in mock treated (8 hours) THP-1 cell extract, and 1735 pg/mL in untreated THP-1 cell extract.

  • The concentrations of Caspase-1 were measured in duplicate and interpolated from the Caspase-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Caspase-1 concentration was determined to be 128.7 pg/mL in thyroid tissue extract and 2279 pg/mL in BxPC-3 cell extract.

  • Undiluted samples are as follows: THP-1 LPS neat, THP-1 mock neat, PBMC PHA-M neat, PBMC mock neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Caspase-1 concentration was determined to be 549 pg/mL in LPS treated (8 hours) THP-1 cell culture supernatant, 41.8 pg/mL in mock treated (8 hours) THP-1 cell culture supernatant, 204 pg/mL PHA-M treated (2 days) PBMC cell culture supernatant, and 57.3 pg/mL in mock treated (2 days) PBMC cell culture supernatant.

  • Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 2 days. The concentrations of Caspase-1 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the Caspase-1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Caspase-1 concentration was determined to be 549 pg/mL in LPS stimulated THP-1 cell supernatant, 41.8 pg/mL in unstimulated THP-1 cell supernatant, 204 pg/mL in PHA-M stimulated PBMC cell supernatant, 57.3 pg/mL in unstimulated PBMC supernatant, and undetectable in media (not shown).

Protocols

References

ab219633 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219633.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up