Overview

  • Product name
    Human Cathepsin B ELISA Kit
    See all Cathepsin B kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin B specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin B amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Cathepsin B measured in culture supernatants from control (-) or stimulated cells cells (P/L: 24 hours with 50 ng x mL-1 of PMA (ab120297) with 1 ug x mL-1 of LPS (Sigma) for the last 6 hours; PHA: 48 hours with 2% PHA-M; LifeTechnologies). Data shown represents duplicates (+/- SD), samples tested in the dilution range of 1/1-1/20.

  • Cathepsin B measured in biological fluids (tested in the range of 1/10-1/1000; duplicates +/- SD).

  • Representative Standard Curve Using ab119584.

Protocols

References

This product has been referenced in:
  • Zhu S  et al. Inhibiting autophagy potentiates the anticancer activity of IFN1@/IFNa in chronic myeloid leukemia cells. Autophagy 9:317-27 (2013). Read more (PubMed: 23242206) »

See 1 Publication for this product

Customer reviews and Q&As



J’ai eu une réponse du laboratoire qui m’a confirmé que les plaques sont divisibles. La plaque peut être divisé en 12 donc vous pouvez tester 8 échantillons à chaque fois.

Une foi...

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Thank you for your reply which has been forwarded to me as my colleague Karin is currently away from the office.

I am very sorry to confirm that there are no discounts available that I can provide on this occasion. I can let you know that if...

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Thank you for contacting us and sorry for the delay in getting back to you.

I can confirm that the Cathepsin B Human ELISA Kit (ab119584) can detect both the mature form and uncleaved form of Cathepasin B.

The kit has been found to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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