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Abcam’s Human Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).
A Cathepsin B specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin B amount of sample captured in plate.
|Components||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-Human Cathepsin B antibody Microplate (12 x 8 wells)||1 unit|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated anti-Human Cathepsin B antibody||1 x 130µl|
|Lyophilized recombinant Human Cathepsin B standard||2 x 10ng|
|Plate Seal||1 x 4 units|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab119584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Cathepsin B measured in culture supernatants from control (-) or stimulated cells cells (P/L: 24 hours with 50 ng x mL-1 of PMA (ab120297) with 1 ug x mL-1 of LPS (Sigma) for the last 6 hours; PHA: 48 hours with 2% PHA-M; LifeTechnologies). Data shown represents duplicates (+/- SD), samples tested in the dilution range of 1/1-1/20.
Cathepsin B measured in biological fluids (tested in the range of 1/10-1/1000; duplicates +/- SD).
Representative Standard Curve Using ab119584.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"