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|Sample type||Average %||Range|
|Serum||92||78% - 113%|
Abcam’s Cathepsin L Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cathepsin L concentrations in cell culture supernatant and serum. This kit is not suitable for the detection of Cathepsin L in plasma.
Cathepsin L specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Cathepsin L detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cathepsin L captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-human Cathepsin L monoclonal antibody||1 x 70µl|
|Human Cathepsin L Standard||2 vials|
|Microplate coated with monoclonal antibody to Cathepsin L (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 200µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|