Human CCL28 ELISA Kit (ab99988)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 27.4 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CCL28 ELISA Kit
See all CCL28/MEC kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 25 pg/ml -
Range
27.4 pg/ml - 20000 pg/ml -
Recovery
> 65 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 67.7 59% - 81% Serum 70.6 60% - 98% Plasma 68.6 59% - 82% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CCL28 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL28 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CCL28 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman CCL28 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CCL28 2 vials CCL28 Microplate (12 strips x 8 wells) 1 x 200µl Recombinant Human CCL28 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. -
Tissue specificity
Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine ligand 28
- C-C motif chemokine 28
- CC chemokine CCL28
see all -
Database links
- Entrez Gene: 56477 Human
- Omim: 605240 Human
- SwissProt: Q9NRJ3 Human
- Unigene: 656904 Human
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab99988 has been referenced in 2 publications.
- Huang G et al. Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells. Sci Rep 6:27152 (2016). PubMed: 27250766
- Yoon JH et al. Plasma CC-chemokine ligand 28 level is correlated with hematopoietic stem cells in human cord blood. Transfusion 55:1008-12 (2015). PubMed: 25412854