• Product nameHuman CCL28 ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    27.4 pg/ml - 20000 pg/ml
  • Recovery

    > 65 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 67.7 59% - 81%
    Serum 70.6 60% - 98%
    Plasma 68.6 59% - 82%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CCL28 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL28 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CCL28 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman CCL28 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceThis protein belongs to the subfamily of small cytokine CC proteins. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. This cytokine displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils and binds to chemokine receptors CCR3 and CCR10. It induces calcium mobilization in a dose-dependent manner. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs.
  • Alternative names
    • CCK 1
    • CCK1
    • CCL 28
    • Chemokine (C-C motif) ligand 28
    • MEC
    • MGC71902
    • Mucosae associated epithelial chemokine
    • OTTHUMP0000022162
    • SCYA28
    • Small inducible cytokine A28
    • Small inducible cytokine A28 [Precursor]
    • Small inducible cytokine subfamily A (Cys-Cys), member 28
    see all
  • Database links


Our Abpromise guarantee covers the use of ab99988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CCL28 ELISA Kit images

  • Representative Standard Curve using ab99988

  • Representative Standard Curve using ab99988


References for Human CCL28 ELISA Kit (ab99988)

This product has been referenced in:
  • Yoon JH  et al. Plasma CC-chemokine ligand 28 level is correlated with hematopoietic stem cells in human cord blood. Transfusion 55:1008-12 (2015). Read more (PubMed: 25412854) »

See 1 Publication for this product

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