Overview

  • Product name
    Human CD130 ELISA Kit (GP130)
    See all CD130 (gp130) kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 1751pg/ml 17.61 1%
    B 8 264.3pg/ml 8.67 3.27%
    Inter-assay
    Sample n Mean SD CV%
    A 16 1782pg/ml 26.64 1.49%
    B 16 247pg/ml 11.99 4.85%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    56.25 pg/ml - 1800 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Serum 97 % - %

  • Assay time
    1h 40m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD130 (GP130) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD130 in Human serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD130 present in the samples.

    This kit will recognize both endogenous and recombinant Human CD130.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 2 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-CD130 Red 1 x 400µl 2 x 400µl
    CD130 Microplate (12 x 8 well strips) 1 unit 2 units
    CD130 Standard (lyophilized) Yellow 2 vials 4 vials
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Relevance
    CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
  • Cellular localization
    Cell membrane; single-pass type I membrane protein and secreted
  • Alternative names
    • CD130 antigen
    • CDw130
    • gp130
    • IL6 R beta
    • Interleukin 6 receptor subunit beta
    • Interleukin 6 signal transducer
    • Membrane glycoprotein 130
    • Oncostatin M receptor alpha subunit
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  •  Representative Standard Curve using ab46135

Protocols

References

ab46135 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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