|Sample type||Average %||Range|
|Serum||97||% - %|
Abcam’s CD130 (GP130) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD130 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD130 present in the samples.
This kit will recognize both endogenous and recombinant Human CD130.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||2 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 7.5ml||1 x 13ml|
|Biotinylated anti-CD130||Red||1 x 400µl||2 x 400µl|
|CD130 Microplate (12 x 8 well strips)||1 unit||2 units|
|CD130 Standard (lyophilized)||Yellow||2 vials||4 vials|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|Control||Silver||2 vials||4 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab46135 has not yet been referenced specifically in any publications.