Overview

  • Product nameHuman CD134 ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 12%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    1.8 pg/ml
  • Range
    78 pg/ml - 5000 pg/ml
  • Recovery

    88 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD134 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD134 concentrations in cell culture supernatant and serum.

    CD134 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated CD134 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD134 captured on the plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionReceptor for TNFSF4/OX40L/GP34.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • ACT35
    • ACT35 antigen
    • CD134
    • CD134 antigen
    • Lymphoid activation antigene ACT35
    • OX40
    • OX40 antigen
    • OX40 cell surface antigen
    • OX40 homologue
    • OX40L receptor
    • TAX transcriptionally-activated glycoprotein 1 receptor
    • TNFRSF4
    • TNR4_HUMAN
    • Tumor necrosis factor receptor superfamily member 4
    • TXGP1L
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CD134 ELISA Kit images

  • Representative Standard Curve using ab119510.

Protocols

References for Human CD134 ELISA Kit (ab119510)

ab119510 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately, the antibodies used in ab119510 ELISA kit have not had their epitopes mapped and thus we are not able to provide you with the binding region.

Thank you for contacting Abcam.

We use different antibodies for this ELISA. Unfortunately which ones we use is proprietary and so I cannot provide anymore information.
If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"