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Abcam’s CD137 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD137 concentrations in cell culture supernatant and serum.
CD137 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD137 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD137 captured in plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-Human CD137 polyclonal antibody||1 x 100µl|
|Human CD137 monoclonal antibody coated microplate (12 x 8 wells)||1 unit|
|Human CD137 Standard, lyophilized (100 ng/mL on reconstitution)||2 vials|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119511 has not yet been referenced specifically in any publications.