Overview

  • Product nameHuman CD137 ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 7.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.9%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.2 ng/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Recovery

    90 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD137 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD137 concentrations in cell culture supernatant and serum.

    CD137 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD137 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD137 captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionReceptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
  • Tissue specificityExpressed on the surface of activated T-cells.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • 4-1BB
    • 4-1BB ligand receptor
    • CD137
    • CD137 antigen
    • CDw137
    • Homolog of mouse 4 1BB
    • ILA
    • Induced by lymphocyte activation (ILA)
    • Interleukin activated receptor homolog of mouse Ly63
    • Receptor protein 4 1BB
    • T-cell antigen 4-1BB homolog
    • T-cell antigen ILA
    • TNFRSF9
    • TNR9_HUMAN
    • Tumor necrosis factor receptor superfamily member 9
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CD137 ELISA Kit images

  • Representative Standard Curve using ab119511.

Protocols

References for Human CD137 ELISA Kit (ab119511)

ab119511 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"