Overview

  • Product nameHuman CD14 ELISA Kit
    See all CD14 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 210.5ng/ml 2.6 1.23%
    Sample B 8 23.4ng/ml 1.14 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 8 210.5ng/ml 1.55 0.73%
    Sample B 8 24 2 8.3%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 ng/ml
  • Range
    6.8 ng/ml - 220 ng/ml
  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD14 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD14 in Human serum, plasma, buffered solutions or cell culture media.

    A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD14 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD14 present in the samples.

    This kit will recognize both endogenous and recombinant Human CD14.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Alternative names
    • CD 14
    • CD_antigen=CD14
    • CD14
    • CD14 antigen
    • CD14 molecule
    • CD14_HUMAN
    • LPS-R
    • Mo2
    • Monocyte differentiation antigen CD14
    • Monocyte differentiation antigen CD14 urinary form
    • Monocyte differentiation antigen CD14, membrane-bound form
    • Myeloid cell specific leucine rich glycoprotein
    • Myeloid cell-specific leucine-rich glycoprotein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab45523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CD14 ELISA Kit images

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted

  • hu CD14 measured in biologicals showing quantity (ng) per mL of tested sample

Protocols

References for Human CD14 ELISA Kit (ab45523)

This product has been referenced in:
  • Malherbe G  et al. Circulating biomarkers of immune activation distinguish viral suppression from nonsuppression in HAART-treated patients with advanced HIV-1 subtype C infection. Mediators Inflamm 2014:198413 (2014). Read more (PubMed: 24808634) »

See 1 Publication for this product

Product Wall


ab45523 should recognize soluble CD14, as it has been tested and is guaranteed to detect the protein in cell culture supernatant, in which only soluble CD14 would be present.

I am sorry for the delay in getting back to you.

As discussed in my previous email, the human CD14 ELISA kits ab99989 and ab45523 are both sandwich ELISA kits which bind CD14 to the surface of the plate and use another antibody to detect the ...

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Thank you for your enquiry. I can suggest the following procedure which can be used to lyse the THP cells ready for CD14 detection using this ELISA kit: Lysis Buffer : Tris 50mM Nacl 150mM 1%NP40 (Nonidet P40) pH=8.0 1-Collect the cells by ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"