Abcam’s CD14 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD14 in Human serum, plasma, buffered solutions or cell culture media.
A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD14 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD14 present in the samples.
This kit will recognize both endogenous and recombinant Human CD14.
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|Components||Identifier||2 x 96 tests||1 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||2 x 10ml||1 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 13ml||1 x 7ml|
|Biotinylated anti-CD14||2 x 0.3ml||1 x 0.3ml|
|CD14 Microplate (12 x 8 well strips)||2 units||1 unit|
|CD14 Standard (lyophilized)||4 vials||2 vials|
|Chromogen TMB Substrate Solution||1 x 24ml||1 x 11ml|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Stop Reagent||Black||2 x 11ml||1 x 11ml|
|Streptavidin-HRP||4 x 5µl||2 x 5µl|
Our Abpromise guarantee covers the use of ab45523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted
hu CD14 measured in biologicals showing quantity (ng) per mL of tested sample