Overview

  • Product name
    Human CD14 ELISA Kit
    See all CD14 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    74 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 73.77 67% - 81%
    Serum 76.23 70% - 85%
    Plasma 73.87 67% - 80%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD14 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD14 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human CD14 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD14 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD14 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD14 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human CD14 ELISA Kit (ab208983) from our SimpleStep ELISA® range.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99989.

Protocols

References

ab99989 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you very much for the clarification. Please see below for the details of your refund.

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I am sorry that thiskit did not perform as stated on the datasheet. If payment has already been made on the origin...

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Thank you again for sending all of this data and for your patience. I am working with my colleagues to set up this refund for you.

I apologize for my confusion regarding the background. I hadn't realized this was a reference wavelength readin...

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Thank you for your emails, and I am sorry to hear that the replacement kits are also not providing the expected results.

Could you tell me how many of the replacement kits were tried, and were the samples and standards both giving low results...

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Thank you again for sending all of the data.

I have heard back from the lab, and they are wondering if you could send the OD values before background subtraction (or just indicate the value of background)? The lab scientists would just like t...

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Thank you for letting me know. I apologize if I forgot to attach the data! Please see the file attached.

I have also received the additional information that you sent in another email, and I will forward that on to the lab. Please let me know...

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Thank you for your calls earlier this week and for your patience while I have been in touch with the lab regarding your enquiry about ab99989.

I have obtained the standard OD values determined during the QC testing for the two lots that you h...

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Thank you for your calls last week and for your patience while I have been in touch with the lab regarding your enquiries. Thank you also for sending your data from the most recent assays with the kits.
I have requested to see the standard curve d...

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Thank you for your call today and for letting us know about the problem with these kits.

As we discussed, I'm sending free of charge replacement kits on the order ***, which I expect to arrive by the beginning of next week.

If you h...

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Thank you for contacting us yesterday in regards to the CD14 Human ELISA Kit (ab99989).

I cannow confirmthe detailsyourequested.We wouldrecommendedusing between a 2,000-20,000 fold dilution for human plasma. Therefore startingwith a 1/5000 di...

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I am sorry for the delay in getting back to you.

As discussed in my previous email, the human CD14 ELISA kits ab99989 and ab45523 are both sandwich ELISA kits which bind CD14 to the surface of the plate and use another antibody to detect the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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