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|Sample type||Average %||Range|
|Cell culture supernatant||73.77||67% - 81%|
|Serum||76.23||70% - 85%|
|Plasma||73.87||67% - 80%|
Abcam’s CD14 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD14 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CD14 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD14 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD14 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD14 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human CD14 ELISA Kit (ab208983) from our SimpleStep ELISA® range.
Optimisation may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-Human CD14||2 vials|
|CD14 Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Human CD14 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab99989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab99989 has not yet been referenced specifically in any publications.