• Product nameHuman CD14 ELISA Kit
    See all CD14 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    74 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 73.77 67% - 81%
    Serum 76.23 70% - 85%
    Plasma 73.87 67% - 80%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD14 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD14 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD14 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD14 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD14 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD14 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
  • Post-translational
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Alternative names
    • CD 14
    • CD_antigen=CD14
    • CD14
    • CD14 antigen
    • CD14 molecule
    • CD14_HUMAN
    • LPS-R
    • Mo2
    • Monocyte differentiation antigen CD14
    • Monocyte differentiation antigen CD14 urinary form
    • Monocyte differentiation antigen CD14, membrane-bound form
    • Myeloid cell specific leucine rich glycoprotein
    • Myeloid cell-specific leucine-rich glycoprotein
    see all
  • Database links


Our Abpromise guarantee covers the use of ab99989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CD14 ELISA Kit images

  • Representative Standard Curve using ab99989.


References for Human CD14 ELISA Kit (ab99989)

ab99989 has not yet been referenced specifically in any publications.

Product Wall

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I apologize for my confusion regarding the background. I hadn't realized this was a reference wavelength readin...

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Thank you for your emails, and I am sorry to hear that the replacement kits are also not providing the expected results.

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Thank you again for sending all of the data.

I have heard back from the lab, and they are wondering if you could send the OD values before background subtraction (or just indicate the value of background)? The lab scientists would just like t...

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Thank you for your calls earlier this week and for your patience while I have been in touch with the lab regarding your enquiry about ab99989.

I have obtained the standard OD values determined during the QC testing for the two lots that you h...

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Thank you for your calls last week and for your patience while I have been in touch with the lab regarding your enquiries. Thank you also for sending your data from the most recent assays with the kits.
I have requested to see the standard curve d...

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Thank you for your call today and for letting us know about the problem with these kits.

As we discussed, I'm sending free of charge replacement kits on the order ***, which I expect to arrive by the beginning of next week.

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Thank you for contacting us yesterday in regards to the CD14 Human ELISA Kit (ab99989).

I cannow confirmthe detailsyourequested.We wouldrecommendedusing between a 2,000-20,000 fold dilution for human plasma. Therefore startingwith a 1/5000 di...

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I am sorry for the delay in getting back to you.

As discussed in my previous email, the human CD14 ELISA kits ab99989 and ab45523 are both sandwich ELISA kits which bind CD14 to the surface of the plate and use another antibody to detect the ...

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