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|Sample type||Average %||Range|
|Serum||= 104.8||97% - 113%|
|Plasma||= 102.1||94% - 110%|
|Cell culture media||= 99.57||93% - 106%|
Abcam’s CD163 (M130) Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human CD163 in serum, plasma (EDTA plasma is not suitable for use in this assay due to its chelating property) and cell culture supernatants.
This assay employs an antibody specific for Human CD163 (M130) coated on a 96-well plate. Standards and samples are pipetted into the wells and CD163 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD163 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD163 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human CD163||2 vials|
|CD163 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human CD163 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab155428 has not yet been referenced specifically in any publications.