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Abcam’s CD21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD21 in Human serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for CD21 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD21 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD21 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD21 present in the samples.
This kit will recognize both endogenous and recombinant Human CD21.
|Components||Identifier||2 x 96 tests||1 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||2 x 10ml||1 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 13ml||1 x 7ml|
|Biotinylated anti-CD21 antibody||Red||2 x 300µl||1 x 300µl|
|CD21 Microplate (12 x 8 well strips)||1 x 2 units||1 unit|
|CD21 Standard||Yellow||4 vials||2 vials|
|Chromogen TMB Substrate Solution||1 x 24ml||1 x 11ml|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Stop Reagent||Black||2 x 11ml||1 x 11ml|
|Streptavidin HRP||4 x 5µl||2 x 5µl|
Our Abpromise guarantee covers the use of ab45909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab45909 has not yet been referenced specifically in any publications.