Overview

  • Product name
    Human CD26 ELISA Kit
    See all CD26 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 9.1%
  • Sample type
    Cell culture supernatant, Serum
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    7.3 ng/ml
  • Range
    15.6 ng/ml - 500 ng/ml
  • Recovery

    101 %

    Sample specific recovery
    Sample type Average % Range
    Serum 101 81% - 116%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD26 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD26 concentrations in cell culture supernatant and serum.

    CD26 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD26 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD26 sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-Human CD26 monoclonal antibody 1 x 70µl
    Human CD26 Standards (500 ng/mL upon dilution) 2 vials
    Microplate coated with monoclonal antibodies to Human CD26 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Function
    Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • Tissue specificity
    Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • Sequence similarities
    Belongs to the peptidase S9B family. DPPIV subfamily.
  • Domain
    The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
  • Post-translational
    modifications
    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
  • Cellular localization
    Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
  • Information by UniProt
  • Alternative names
    • CD26 antigen
    • ADA-binding protein
    • ADABP
    • ADCP 2
    • ADCP-2
    • ADCP2
    • Adenosine deaminase complexing protein 2
    • CD 26
    • CD26
    • CD26 antigen 3
    • Dipeptidyl peptidase 4
    • Dipeptidyl peptidase 4 soluble form
    • Dipeptidyl peptidase IV
    • Dipeptidyl peptidase IV membrane form
    • Dipeptidyl peptidase IV soluble form
    • Dipeptidyl peptidase, intestinal
    • Dipeptidylpeptidase 4
    • Dipeptidylpeptidase IV
    • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
    • DPP 4
    • DPP IV
    • DPP IV estoenzyme
    • DPP4
    • DPP4_HUMAN
    • DPPIV
    • Intestinal dipeptidyl peptidase
    • T cell activation antigen CD26
    • T-cell activation antigen CD26
    • TP 103
    • TP103
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119513.

Protocols

References

ab119513 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I am afraid but our CD26 Human ELISA Kit (with color giving dyes) ab119513 is completely different from the DPPIV drug discovery kit, and the decision for either product depends on what you would like to investiga...

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