Overview

  • Product nameHuman CD28 ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.18 ng/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Recovery

    117 %

    Sample specific recovery
    Sample type Average % Range
    Serum 105 90% - 130%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD28 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD28 concentrations in cell culture supernatant and serum.

    CD28 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD28 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD28 sample captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • CD28
    • CD28 antigen
    • CD28 molecule
    • CD28_HUMAN
    • T-cell-specific surface glycoprotein CD28
    • TP44
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CD28 ELISA Kit images

  • Representative Standard Curve using ab119514.

References for Human CD28 ELISA Kit (ab119514)

ab119514 has not yet been referenced specifically in any publications.

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