Overview

  • Product nameHuman CD31 ELISA Kit (PECAM1)
    See all CD31 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 11 5.323ng/ml 0.19 3.5%
    Sample B 11 1.236ng/ml 0.077 6.2%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 11 5.406ng/ml 0.292 5.4%
    Sample B 11 1.205ng/ml 0.058 4.8%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.17 ng/ml
  • Range
    0.31 ng/ml - 10 ng/ml
  • Assay time
    2h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD31 (PECAM1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD31 in Human serum, plasma, buffered solutions and cell culture medium

    A monoclonal antibody specific for CD31 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD31 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD31 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD31 present in the samples.

    This kit will recognize both endogenous and recombinant Human CD31.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • Post-translational
    modifications
    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • Alternative names
    • CD31
    • CD31 antigen
    • EndoCAM
    • GPIIA
    • GPIIA''
    • HEC7
    • Leukocyte surface protein
    • PECA1
    • PECA1_HUMAN
    • PECAM 1
    • PECAM-1
    • Pecam1
    • Platelet endothelial cell adhesion molecule
    • Platelet/endothelial cell adhesion molecule
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab45910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CD31 ELISA Kit (PECAM1) images

  • Representative Standard Curve using ab45910.

References for Human CD31 ELISA Kit (PECAM1) (ab45910)

This product has been referenced in:
  • Chen R  et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . Read more (PubMed: 20885780) »

See 1 Publication for this product

Product Wall



Le confirmo que ambos kits detectan la parte soluble de PECAM1. La principal diferencia que presentan es la sensibilidad. ab45910 cubre el rango comprendido entre 0.31 ng/ml - 10 ng/ml, mientras que ab100627 es más sensible, abarcando ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"