Abcam’s CD40 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD40 concentrations in cell culture supernatant, serum, plasma (EDTA, heparin) and amniotic fluid.
CD40 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD40 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD40 sample captured in plate.
CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
B cell associated molecule
B cell associated molecule CD40
B cell surface antigen CD40
CD40 antigen (TNF receptor superfamily member 5)
CD40 molecule TNF receptor superfamily member 5
CD40 type II isoform
Nerve growth factor receptor related B lymphocyte activation molecule
TNF receptor superfamily member 5
Tumor necrosis factor receptor superfamily member 5
Tumor necrosis factor receptor superfamily member 5 precursor
Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a