|Sample type||Average %||Range|
|Serum||94||81% - 110%|
Abcam’s CD40 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD40 concentrations in cell culture supernatant, serum, plasma (EDTA, heparin) and amniotic fluid.
CD40 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD40 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD40 sample captured in plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-Human CD40 monoclonal antibody||1 x 70µl|
|CD40 Standard Lyophilized (1000 pg/mL on reconstitution)||2 vials|
|Microplate coated with anti-Human CD40 monoclonal antibody (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119516 has not yet been referenced specifically in any publications.