|Sample type||Average %||Range|
|Serum||92||84% - 100%|
Abcam’s CD44var (v5) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD44var (v5) concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid and urine.
CD44var (v5) specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells, along with a CD44var (v5) HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the CD44var (v5) amount of sample captured in plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||2 units|
|HRP-Conjugate anti-Human CD44var (v5) monoclonal antibody||2 vials|
|Human CD44var (v5) Standard Lyophilized (20 ng/mL on reconstitution)||2 vials|
|Microplate coated with monoclonal anti-Human CD44var (v5) antibodies (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 50ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119518 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119518 has not yet been referenced specifically in any publications.