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|Sample type||Average %||Range|
|Serum||106||89% - 115%|
Abcam’s CD44var (v6) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD44var (v6) concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), and urine.
CD44var (v6) specific antibodies have been precoated onto a 96-well plate. Standards and test samples are added to the wells and along with a CD44var (v6) HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the CD44var (v6) amount of sample captured in plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||2 units|
|HRP-Conjugate anti-Human CD44var (v6) monoclonal antibody||2 x 10µl|
|Human CD44var (v6) Standard Lyophilized 20 ng/mL upon reconstitution)||2 vials|
|Microplate coated with anti CD44var (v6) monoclonal antibody (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 50ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119519 has not yet been referenced specifically in any publications.