• Product name
    Human CD62E ELISA Kit (Selectin)
    See all CD62E kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.39 83% - 103%
    Serum 93.75 83% - 102%
    Plasma 95.38 84% - 105%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD62E (Selectin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62E in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD62E coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Function
    Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • CD62 antigen-like family member E
    • CD62E
    • CD62E antigen
    • E selectin
    • E-selectin
    • ELAM
    • ELAM-1
    • ELAM1
    • Endothelial leukocyte adhesion molecule 1
    • ESEL
    • LECAM2
    • Leukocyte endothelial cell adhesion molecule 2
    • Leukocyte-endothelial cell adhesion molecule 2
    • Sele
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CD62E ELISA Kit (Selectin) images

  • Representative Standard Curve using ab100512.

  • Representative Standard Curve using ab100512.


References for Human CD62E ELISA Kit (Selectin) (ab100512)

This product has been referenced in:
  • Chitalia N  et al. Impact of vitamin D supplementation on arterial vasomotion, stiffness and endothelial biomarkers in chronic kidney disease patients. PLoS One 9:e91363 (2014). Read more (PubMed: 24646518) »

See 1 Publication for this product

Product Wall

Yes, I can confirm that EDTA, Heparin, or Citrate collected plasma can be used with the ab100512 CD62E Human ELISA Kit

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not...

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Thank you for your inquiry and I apologize for the delay while I was awaiting more information from our testing laboratory. There is a way that you can to test for TMB and HRP-streptavidin activity: Prepare a 100-fold dilution of HRP-Streptavidin...

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