The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
CD74 molecule, major histocompatibility complex, class II invariant chain
Gamma chain of class II antigens
HLA class II histocompatibility antigen gamma chain
HLA DR antigens associated invariant chain
HLA DR gamma
HLA-DR antigens-associated invariant chain
HLADR antigens associated invariant chain
Ia antigen associated invariant chain
Ia antigen-associated invariant chain
Ia associated invariant chain
Invariant polypeptide of major histocompatibility complex class II antigen associated
Major histocompatibility complex class II invariant chain
MHC HLA DR gamma chain
MHC HLA-DR gamma chain
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Contains 1 thyroglobulin type-1 domain.
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.