Overview

  • Product name
    Human CD86 ELISA Kit
    See all CD86 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 37.25U/ml 0.329 0.88%
    Sample B 8 5.09U/ml 0.193 3.79%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 39 37.63U/ml 0.611 1.62%
    Sample B 39 4.92U/ml 0.348 7.06%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.6 U/ml
  • Range
    1.187 U/ml - 38 U/ml
  • Assay time
    2h 40m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human CD86 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD86 in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD86 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD86.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab45921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • CD86 in U937 lysates from control cells or cells stimulated for 24 hours with 50 ng x mL-1 PMA (ab120297) or PMA with 1 ug x mL-1 LPS for the last 6 hours (duplicates; +/- SD).

    2e6 snap frozen cells were lysed in 0.3 mL RIPA lysis buffer on ice, sonicated in an ice cold water bath for 12 intervals for a total of 3.5 minutes. The supernatants were cleared by centrifugation (max speed, 10 minutes, 4 degrees), and tested undiluted or diluted 1/4.

  • CD86 measured in undiluted 72 hours culture supernatants from control of treated (5 ug x mL-1 PHA; Sigma) PBMCs (1e6 cells x mL-1; duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab45921

Protocols

References

ab45921 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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