Overview

  • Product nameHuman CD95 ELISA Kit (TNFRSF6)
    See all CD95 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 14 1719pg/ml 106 6.1%
    Sample B 16 703pg/ml 39 5.5%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 8 1752pg/ml 145 8.2%
    Sample B 8 856pg/ml 68 7.9%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 47 pg/ml
  • Range
    93.75 pg/ml - 3000 pg/ml
  • Assay time
    1h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human soluble CD95 (TNFRSF6) ELISA is to be used for the in-vitro quantitative determination of soluble CD95 in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for CD95 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD95 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD95 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD95 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD95.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionReceptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • Tissue specificityIsoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • Involvement in diseaseDefects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similaritiesContains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • DomainContains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • ALPS1A
    • APO 1
    • APO 1 cell surface antigen
    • Apo-1 antigen
    • APO1
    • Apo1 antigen
    • Apoptosis antigen 1
    • Apoptosis mediating surface antigen FAS
    • Apoptosis-mediating surface antigen FAS
    • APT1
    • CD95
    • CD95 antigen
    • Fas
    • Fas AMA
    • Fas antigen
    • FAS1
    • FASLG receptor
    • FASTM
    • TNF receptor superfamily member 6
    • TNFRSF6
    • TNR6_HUMAN
    • Tumor necrosis factor receptor superfamily member 6
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab45922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CD95 ELISA Kit (TNFRSF6) images

  • Representative Standard Curve using ab45922

Protocols

References for Human CD95 ELISA Kit (TNFRSF6) (ab45922)

ab45922 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

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These ELISA kits are from two different sources but they are similar.

I recommend todetermine which is the best foryour needs from the datasheet and protocol information. Please take not of the different ...

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Thank for your quick response. As I have noted in my previous e-mail, the kit seems to be working since the standard curve obtained is fine. The actual standard values are concentration-dependent and the curve is linear. We assayed over 50 health...

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Thank for your enquiry regarding ab45922 and for providing some data saved in Excel file. After reviewing the results you have kindly sent to us, the standard curve obtained is fair and it seems the kit worked fine. Could you please confirm t...

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