The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Caudal type homeo box 2
Caudal type homeo box transcription factor 2
Caudal type homeobox 2
Caudal type homeobox protein 2
Caudal type homeobox transcription factor 2
Caudal-type homeobox protein 2
Homeobox protein CDX 2
Homeobox protein CDX-2
FunctionInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Sequence similaritiesBelongs to the Caudal homeobox family. Contains 1 homeobox DNA-binding domain.
Post-translational modificationsPhosphorylation of Ser-60 mediates the transactivation capacity.
Madeja ZE et al. Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development. BMC Dev Biol13:32 (2013).
Read more (PubMed: 23941255) »