• Product nameHuman CEACAM1 ELISA Kit (BGP)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    20.58 pg/ml - 15000 pg/ml
  • Recovery

    > 83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 83.49 75% - 90%
    Serum 96.92 82% - 112%
    Plasma 86.5 70% - 105%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CEACAM1 (BGP) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEACAM1 in serum, plasma, cell culture supernatant.

    This assay employs an antibody specific for Human CEACAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CEACAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEACAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEACAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    400X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human CEACAM1 2 vials
    CEACAM1 Microplate (12 x 8 well strips) 1 unit
    Recombinant Human CEACAM1 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • RelevanceCEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
  • Alternative names
    • Antigen CD66
    • BGP
    • BGP 1
    • BGP1
    • BGPI
    • Biliary glycoprotein
    • Biliary glycoprotein 1
    • Biliary glycoprotein adhesion molecule
    • Carcinoembryonic antigen related cell adhesion molecule 1
    • CD66a
    • CEA related cell adhesion molecule
    • CEACAM 1
    • CEACAM1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab99993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human CEACAM1 ELISA Kit (BGP) images

  • Representative Standard Curve using ab99993.

  • Representative Standard Curve using ab99993.


References for Human CEACAM1 ELISA Kit (BGP) (ab99993)

ab99993 has not yet been referenced specifically in any publications.

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