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|Sample type||Average %||Range|
|Cell culture supernatant||83.49||75% - 90%|
|Serum||96.92||82% - 112%|
|Plasma||86.5||70% - 105%|
Abcam’s CEACAM1 (BGP) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEACAM1 in serum, plasma, cell culture supernatant.
This assay employs an antibody specific for Human CEACAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CEACAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEACAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEACAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human CEACAM1||2 vials|
|CEACAM1 Microplate (12 x 8 well strips)||1 unit|
|Recombinant Human CEACAM1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab99993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab99993 has not yet been referenced specifically in any publications.