Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab39846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Blocking - Blocking peptide for Anti-Centaurin gamma 1 antibody (ab21271)

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS, pH 7.2

General Info

  • Alternative names
    • AGAP-2
    • Agap2
    • Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
    • AGAP2_HUMAN
    • ANK repeat and PH domains 2
    • Arf GAP with GTP binding protein like
    • Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2
    • Arf GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2
    • Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
    • ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
    • centaurin gamma1
    • Centaurin-gamma-1
    • Centg1
    • cnt g1
    • Cnt-g1
    • Cnt2
    • FLJ16430
    • GGAP2
    • GTP binding and GTPase activating protein 2
    • GTP-binding and GTPase activating protein 2
    • GTP-binding and GTPase-activating protein 2
    • KIAA0167
    • mKIAA0167
    • Nuclear GTPase PIKE
    • Phosphatidylinositol 3-kinase enhancer
    • Phosphoinositide 3 kinase enhancer
    • PI3K ENHANCER
    • PIKE
    see all
  • Function
    GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
  • Tissue specificity
    Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
  • Sequence similarities
    Belongs to the centaurin gamma-like family.
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
  • Domain
    G domain binds GTP and has GTPase activity.
    Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
    Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.
  • Post-translational
    modifications
    Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.
  • Cellular localization
    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt

References

ab39846 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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