Human cervix tissue lysate (adenosquamous cell carcinoma) - insoluble fraction (female, 45 years) (ab43957)

Overview

  • Product nameHuman cervix tissue lysate (adenosquamous cell carcinoma) - insoluble fraction (female, 45 years)
    See all Cervix lysates
  • Description
    Hu cervix insoluble fraction lysate
  • General notesTissue: Cervix uteri Location: Cervix Diagnosis: Adeno-squamous cell cervical carcinoma, poorly differentiated. Stage: I T1B2NxM0 Grade: 3 Sex: Female Age: 45 years Insoluble protein fraction


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43957: Extraction 1: soluble protein fraction ab43955 Human Cervix Tumor Tissue lysate ab43954 Human Cervix Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43957 Human Cervix Tumor Tissue lysate ab43956 Human Cervix Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.77% DTT, 0.1% SDS, 30.03% Urea, 15.22% Thiourea, 100% Laemmli buffer
    Note: 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human cervix tissue lysate (adenosquamous cell carcinoma) - insoluble fraction (female, 45 years) (ab43957)

    ab43957 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"