Human Anti-Chlamydia pneumoniae IgG ELISA Kit (ab108721)

Overview

  • Product nameHuman Anti-Chlamydia pneumoniae IgG ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 20 7.2%
    Pos. Serum 24 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 12 10.6%
    Pos. Serum 12 5.2%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Chlamydia pneumoniae IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Chlamydia pneumoniae in Human serum and plasma.

     

    A 96-well plate has been precoated with Chlamydia pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Chlamydia pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia pneumoniae IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108721 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent concentration.

    Protocols

    References for Human Anti-Chlamydia pneumoniae IgG ELISA Kit (ab108721)

    ab108721 has not yet been referenced specifically in any publications.

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